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electroporation instrument  (Bio-Rad)


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    Structured Review

    Bio-Rad electroporation instrument
    Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
    Electroporation Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electroporation+instrument/pmc12914205-114-0-3?v=Bio-Rad
    Average 97 stars, based on 5746 article reviews
    electroporation instrument - by Bioz Stars, 2026-07
    97/100 stars

    Images

    1) Product Images from "Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei"

    Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104353

    Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
    Figure Legend Snippet: Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

    Techniques Used: Plasmid Preparation, Transformation Assay, Marker, Amplification, Electroporation, Homologous Recombination, Selection, Mutagenesis

    Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.
    Figure Legend Snippet: Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

    Techniques Used: Clone Assay, Transformation Assay, Electroporation, Negative Control, Positive Control



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    Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by <t>electroporation;</t> Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.
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    Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

    Journal: STAR Protocols

    Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

    doi: 10.1016/j.xpro.2026.104353

    Figure Lengend Snippet: Overview of the pKD46-Red-mediated scarless deletion of the target gene in S. sonnei ① The pKD46 plasmid (encoding Red recombinase) is pre-transformed into S. sonnei and cells are grown at 30 °C to competence. ② A PCR product containing antibiotic-resistance marker flanked by two FRT sites is amplified from pKD3/pKD4. ③ The targeting fragment is introduced by electroporation; Red-driven homologous recombination and antibiotic selection yield primary recombinants. ④ Cultivation at 42 °C cures the temperature-sensitive pKD46. ⑤ Introduction of the CP20 plasmid (encoding FLP recombinase) excises the resistance cassette between the FRT sites; a second 42 °C heat step eliminates CP20, leaving a clean, marker-free deletion mutant. H1/H2, homology extensions; P1/P2, primer sites; FRT, FLP recognition target. The figure is reprinted with permission from Wang et al., 2025. Created with BioRender.

    Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

    Techniques: Plasmid Preparation, Transformation Assay, Marker, Amplification, Electroporation, Homologous Recombination, Selection, Mutagenesis

    Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

    Journal: STAR Protocols

    Article Title: Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

    doi: 10.1016/j.xpro.2026.104353

    Figure Lengend Snippet: Identification of the interaction between CRP and YdeH using the BACTH system Both crp and ydeH were cloned and inserted into the pUT18C and pKNT25 vectors, respectively, and co-transformed into the BTH101 strain by electroporation. The positive clones were spotted together with the negative control and positive control on the colour plate. Finally, the colour development of the plate was checked. Figure reprinted with permission from Wang et al., 2025. Created with BioRender.

    Article Snippet: Electroporation instrument , BIO-RAD , Gene Pulser Xcell.

    Techniques: Clone Assay, Transformation Assay, Electroporation, Negative Control, Positive Control